Cellartis mesenchymal stem cell xeno-free culture medium

Mesenchymal stem cells (MSCs) are multipotent cells that can differentiate into neurons, hepatocytes, pancreatic islet cells, adipocytes, chondrocytes, and osteoblasts, both in vivo and in vitro, and their self-renewal capacity is easily lost by long-term culture and excessive passages. Cellartis MSC Xeno-Free Culture Medium provides optimal conditions that enable maintenance of MSC proliferation and multipotency without the need for plate coating reagents. This medium does not contain any animal-derived components. It is also available in a phenol-red-free version and a GMP-grade version.

Cat. #	Product	Size	Price
Y50200	Cellartis® MSC Xeno-Free Culture Medium	Each	USD $571.00
Cellartis MSC Xeno-Free Culture Medium is suitable for the maintenance and expansion of human mesenchymal stem cells (MSC) from bone marrow, adipose tissue, or umbilical cord—without plate coating reagents. However, coating cell culture vessels with human fibronectin can further promote proliferation. This xeno-free medium is ideal for cell therapy research applications such as bone/cartilage diseases, bone marrow transplants/GVHD, cardiovascular disease, autoimmune disease, liver disease, and cancer. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back Cellartis xeno-free MSC medium: robust proliferation even without coating hMSCs derived from bone marrow, adipose and umbilical cord were maintained in Cellartis MSC Xeno-Free Culture Medium or a competitor's xeno-free medium, with and without coating (fibronectin), on regular tissue culture plates. Cells grew better for five passages in Cellartis MSC Xeno-Free Culture Medium than in the competitor's medium. MSCs could not attach on the regular tissue culture plate after two or three passages in the competitor's medium without coating. Back Cellartis xeno-free MSC medium maintains healthy MSC phenotype hMSCs derived from bone marrow (BM), adipose (AD), and umbilical cord (UC) were maintained in Cellartis MSC Xeno-Free Culture Medium or a competitor's xeno-free medium, w/ and w/o coating (fibronectin) on regular tissue culture plates. After both 1 and 5 passages, % of CD90+, CD105+ and CD73+ MSCs are identified by FACS analysis. After 5 passages (24 days), Cellartis MSC Xeno-Free Culture Medium maintained a high percentage of cells that were positive for typical MSC markers. Back Cellartis xeno-free MSC medium maintains MSC multilinear differentiation ability hMSCs derived from bone marrow (Panel A) were successfully differentiated into osteoblast (Panel B), adipocyte (Panel C), and chondrocyte (Panel D) following culture in Cellartis MSC Xeno-Free Culture Medium without coating reagent. Back No numerical or structural abnormalities identified in hMSC cultured in Cellartis MSC Xeno-Free Culture Medium Karyotype analysis of hMSC after 5 passages in Cellartis MSC Xeno-Free Culture Medium (coating agent free) identified no numerical or structural abnormalities Back Healthy morphology and growth kinetics of hMSCs cultured in Cellartis MSC Xeno-Free Culture Medium Panel A. Morphology of hMSCs (adipose tissue derived) after three passages in Cellartis MSC Xeno-Free Culture Medium. Cells reached confluency by day 3 with healthy proliferation. Scale bar = 1 mm. Panel B. Growth curve shows hMSCs could be expanded with healthy proliferation until passage 15 in Cellartis MSC Xeno-Free Culture Medium (customer-provided data). Back Y50200: Cellartis MSC Xeno-Free Culture Medium
Y50205	Cellartis® MSC Xeno-Free Culture Medium (w/o Phenol Red)	500 mL	USD $571.00
Cellartis MSC Xeno-Free Culture Medium (w/o Phenol Red) is a xeno-free medium suitable for human MSC culture. It does not contain components, such as BSA, etc., that are derived from non-human species. Furthermore, it enables maintenance of MSC proliferation and pluripotency without plate coating reagents. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back No numerical or structural abnormalities identified in hMSC cultured in Cellartis MSC Xeno-Free Culture Medium Karyotype analysis of hMSC after 5 passages in Cellartis MSC Xeno-Free Culture Medium (coating agent free) identified no numerical or structural abnormalities Back Healthy morphology and growth kinetics of hMSCs cultured in Cellartis MSC Xeno-Free Culture Medium Panel A. Morphology of hMSCs (adipose tissue derived) after three passages in Cellartis MSC Xeno-Free Culture Medium. Cells reached confluency by day 3 with healthy proliferation. Scale bar = 1 mm. Panel B. Growth curve shows hMSCs could be expanded with healthy proliferation until passage 15 in Cellartis MSC Xeno-Free Culture Medium (customer-provided data). Back Y50205: Cellartis MSC Xeno-Free Culture Medium (w/o Phenol Red)
Y50210	Cellartis® MSC Xeno-Free GMP Grade Culture Medium	Each	USD $742.00
Cellartis MSC Xeno-Free GMP Grade Culture Medium is a xeno-free medium suitable for human MSC culture. It does not contain components, such as BSA, etc., that are derived from non-human species and is manufactured as a quality-assured product, in compliance with GMP guidelines of PIC/S (Pharmaceutical Inspection Convention and Pharmaceutical Inspection Co-operation Scheme). Furthermore, it enables maintenance of MSC proliferation and pluripotency without plate-coating reagents. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components

Overview

Stable MSC phenotype—maintain expression of CD markers and multilineage differentiation ability
Robust proliferation—maintain MSC proliferation and rapidly grow cells for several passages
Flexibility and ease of use—maintain MSCs on regular tissue culture plates without coating reagent
Assay compatibility—use our phenol red-free version for spectrophotometric and fluorescence-based assays
Clinical research—use our GMP-grade medium for your cell therapy applications

NOTE: Refer to the images under the Image Data tab (for Cat. # Y50200) in the product table above.

More Information

Applications

Maintenance and expansion of human MSCs derived from bone marrow, adipose tissue, or umbilical cord
Ideal for cell therapy research applications such as:
- Bone and cartilage diseases, BMT, and GVHD
- Cardiovascular diseases
- Autoimmune diseases
- Liver diseases
- Cancer

Additional product information:

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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Cellartis mesenchymal stem cell xeno-free culture medium

Notice to purchaser

Cellartis xeno-free MSC medium: robust proliferation even without coating

Cellartis xeno-free MSC medium maintains healthy MSC phenotype

Cellartis xeno-free MSC medium maintains MSC multilinear differentiation ability

No numerical or structural abnormalities identified in hMSC cultured in Cellartis MSC Xeno-Free Culture Medium

Healthy morphology and growth kinetics of hMSCs cultured in Cellartis MSC Xeno-Free Culture Medium

Y50200: Cellartis MSC Xeno-Free Culture Medium

Notice to purchaser

No numerical or structural abnormalities identified in hMSC cultured in Cellartis MSC Xeno-Free Culture Medium

Healthy morphology and growth kinetics of hMSCs cultured in Cellartis MSC Xeno-Free Culture Medium

Y50205: Cellartis MSC Xeno-Free Culture Medium (w/o Phenol Red)

Notice to purchaser

Overview

More Information

Applications

Additional product information: